High fidelity qPCR

High Fidelity Interval Assignment

Quadrilateral meshing algorithms impose certain constraints on the number of intervals or mesh edges of the curves bounding a surface. When constructing a conformal mesh of a collection of adjoining surfaces, the constraints for all of the surfaces must be simultaneously satisfied. These constraints can be formulated as an integer linear program. Not all solutions to this problem are equally de...

High Fidelity Imaging

High Fidelity Scan Merging

For each scanned object 3D triangulation laser scanners deliver multiple sweeps corresponding to multiple laser motions and orientations. The problem of aligning these scans has been well solved by using rigid and, more recently, non-rigid transformations. Nevertheless, there are always residual local offsets between scans which forbid a direct merging of the scans, and force to some preliminar...

High-throughput microfluidic single-cell RT-qPCR.

A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run. Our device executes all steps of single-cell processing, including cel...

preliminary expression of high fidelity pfu dna polymerase encoding fragment